Wako Product Name
HR Series NEFA-HR(2)
For In Vitro Diagnostic Use
- Detects a variety of Free Fatty Acids
Serum NEFA Recovery Study Free Fatty Acid (NEFA) Activity % Recovery % Acetic acid 0 0 Proprionic acid 0 0 Butyric acid 0 0 Valeric acid 14 9 Caproic acid 98 99 Caprylic acid 97 94 Capric acid 89 86 Lauric acid 98 97 Myristic acid 103 93 Palmitic acid 97 94 Stearic acid 90 - Arachidic acid 94 - Palmitoleic acid 100 103 Oleic acid 100 102 Linoleic acid 99 102 Linolenic acid 102 100 Arachidonic acid 89 85 Citric acid 0 0 Oxalic acid 0 0
- Minimal interferences from other serum components such as uric acid, glucose, hemoglobin, and Bilirubin.
- Specific to Free Fatty Acids with no interferences from other related compounds.
Extraction methods are widely used for the colorimetric determination of non-esterified fatty acids (NEFA) in serum. NEFA are converted to their copper salts that are extracted into an organic solvent. The salts are then complexed with a dye for purposed of colorimetric measurement. Alternatively, extracted NEFA are titrated with standard alkali to an acid base indicator endpoint. These approaches are time consuming, hazardous and not easily automated. Wako has made extensive studies of NEFA quantitation and has succeeded in developing an original enzymatic method which is available in a series of individual reagents. This enzymatic method is accurate, precise, simple and fast. The need for an extraction step has been eliminated and the method can be automated.
The Wako enzymatic method relies upon the acylation of coenzyme A (CoA) by the fatty acids in the presence of added acyl-CoA synthetase (ACS). The acyl-CoA thus produced is oxidized by added acyl-CoA oxidase (ACOD) with generation of hydrogen peroxide, in the presence of peroxidase (POD) permits the oxidative condensation of 3-methy-N-ethyl-N(β-hydroxyethyl)-aniline (MEFA) with 4-aminoantipyrine to form a purple colored adduct which can be measured colorimetrically at 550 nm.
|Intended Use||The Wako HR series NEFA-HR(2) is an in vitro enzymatic colorimetric method assay for the quantitative determination of non-esterified fatty acids (NEFA) in serum.|
|Assay Linearity||0.01-4.00 mEq/L|
Sample: 4 µL
R1: 225 µL
R2: 75 µL
Wavelength: primary: 560 nm, secondary: 670 nm
|Package Insert||HR Series NEFA-HR(2)|
- To request an analyzer application email: firstname.lastname@example.org. (Please include the product name and name of analyzer/instrument)
|Catalog No.||Product Name||Pkg Size||Storage|
|999-34691||HR Series NEFA-HR(2) Color Reagent A||4 x for 50 mL||2-10°C|
|995-34791||HR Series NEFA-HR(2) Solvent A||4 x 50 mL||2-10°C|
|991-34891||HR Series NEFA-HR(2) Color Reagent B||4 x for 25 mL||2-10°C|
|993-35191||HR Series NEFA-HR(2) Solvent B||4 x 25 mL||2-10°C|
|276-76491||NEFA Standard Solution|
|997-76491||Wako NEFA Linearity Set|